Technologies
Currently the Bulgarian center for advanced microscopy includes the following equipment:
I. Andor Revolution XD spinning disc confocal microscope system for high-resolution live cell imaging, equipped with FRAP and laser micro-irradiation, that includes:
1. Nikon TiE Inverted Microscope with Autofocus System
2. Yokogawa CSU-X1 spinning disk
3. Microscope stage automated in X, Y and Z directions. Movement in the Z direction is accomplished by a piezo element
4. Three solid state lasers with wavelengths of 405 nm, 488 nm and 561 nm
5. FRAPPA unit for Fluorescence recovery after photobleaching (FRAP) and photoactivation (PA)
6. TuCam module that allows simultaneous imaging of two different wavelengths
7. Two iXon3 897 EMCCD Cameras
8. Borealis
9. Optical anti-vibration table
10. Computer configuration for controlling the microscope system
11. Cell incubator for the microscope
12. CO2 controller that maintains proper CO2 concentration in the cell incubator
13. Temperature controller that maintains proper temperature in the cell incubator
14. Micropoint laser micro-irradiation module
15. IQ3 software for controlling the components of the microscope system, spinning disk, micro-irradiation module and FRAP module
16. MetaMorph software for controlling the components of the microscope system and for image analysis
II. Zeiss Axiovert 200M inverted motorized epifluorescence microscope that includes:
1. Axiovert 200M inverted motorized epifluorescence microscope
2. Apotom
3. Axiocam camera
4. Anti-vibration table
5. Axiovision, software for controlling the components of the microscope system and for image analysis
III. Andor Dragonfly 505 microscopy system for:
1. Multi-Point Confocal, Widefield and TIRF microscopy
2. ClearView-GPU Deconvolution, SRRF-Stream super-resolution, dSTORM super-resolution
3. Andor Mosaic 3 Duet for FRAP и photomanipulation
4. Andor MicroPoint Galvo – laser micro-irradiation
5. Andor ILE-400 laser combiner with Borealis
6. 405 nm, 488 nm, 561 nm, 637 nm solid-stаte lasers
7. Okolab Bold Line Cage CO2 incubator
8. Inverted microscope ECLIPSE Ti2-E
9. Perfect Focus 4 autofocus system
10. Andor iXon Ultra 888 EMCCD camera
11. Andor Zyla 4.2 Plus CMOS camera
12. CoolLED pE300 epi-fluorescents for DAPI, GFP, RFP
13. CoolLED pE100
14. Fusion software package
15. IQ3 software package
IV. STELLARIS 8 STED Microscopes
1. Laser confocal scanning microscopy
2. White laser (excitation from 440 nm up to 790 nm)
3. Power HyD S, HyD X, and HyD R detectors
4. Stimulated emission depletion (STED) super-resolution microscopy
5. FALCON: Fluorescence lifetime imaging microscopy (FLIM)
6. TauSTED Stimulated emission depletion microscopy with FLIM
7. Fluorescence Correlation Spectroscopy (FCS)
8. Fluorescence Resonance Energy Transfer (FRET) Microscopy
9. Lightning (adaptive deconvolution)
10 FRAP
11. Optical anti-vibration table
12. Computer configuration for controlling the microscope system
13. Cell incubator for the microscope
14. CO2 and O2 controller that maintains proper CO2 and O2 concentration in the cell incubator
15. Temperature controller that maintains proper temperature in the cell incubator
16. Leica Application Suite X (LAS X) software
V. ZEISS LSM 980 with Airyscan 2
1. laser confocal scanning microscopy
2. 36 simultaneous channels (GASP) detector
3. 405, 488, 561 and 594 laser lines.
4. airyscan super-resolution
5. FRAP
6. UV micro-irradiation
7. Optical anti-vibration table
8. Computer configuration for controlling the microscope system
9. Cell incubator for the microscope
10. CO2 and O2 controller that maintains proper CO2 and O2 concentration in the cell incubator
11. Temperature controller that maintains proper temperature in the cell incubator
12. Zen blue software
VI. PicoQuant FLIM for ZEISS LSM 980
1. Rapid FLIM HiRes
2. 488 and 561 pulse lasers
3. PMA Hybrid detector and SPAD (single photon avalanche diode) detector.
4. TCSPC Electronics (MultiHarp 150)
5. Pulsed Interleaved Excitation (PIE)
6. Fluorescence Anisotropy (Polarization)
7. Fluorescence Correlation Spectroscopy (FCS)
8. Fluorescence Resonance Energy Transfer (FRET) Microscopy
9. SymPhoTime 64 Analysis software
VII. ZEISS SteREO Discovery.V20
VIII. Image analysis software:
1. Imaris
2. CellTool
3. ImageJ
4. Fiji